March 29, 2023
Maize genetic transformation is technically challenging and resource demanding. It can take many months when using the traditional protocol. Masters et al. reported a QuickCorn protocol to further simplify maize transformation by removing the callus induction step that is usually necessary. The following video demonstrates this QuickCorn protocol, which can be used by researchers with minimum training on maize transformation and only takes five to seven weeks to become rooted and transferable. This Agrobacterium-mediated transformation has the potential to grow the list of transformable genotypes and may possibly be used with other species.